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HPLC or SFC? Which One Should I Choose?
November 2023
High- Performance Liquid Chromatography (HPLC) and Supercritical Fluid Chromatography (SFC) are both highly effective separation techniques. SFC has been on the rise since its discovery in the 1960s dueto the advantages it gives the user over HPLC. SFC users experience greater resolving strength in chiral separations, shorter run times, less solvent consumption, and lower energy costs. Yet there are still times when HPLC might be the better choice over SFC.
During the webinar you will learn: - What Supercritical Fluid Chromatography is
- How HPLC and SFC differ
- How much time and solvent can be saved using SFC over HPLC
- Why SFC is favored for chiral separations
- When to choose HPLC over SFC
Speaker: Dylan Cavey, Teledyne ISCO Applications Chemist
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HPLC Troubleshooting and Maintenance Techniques
October 2023
Peaks not coming out where they used to? Not as big as they usually are and don’t look the way they should? These are the clear indicators that something in the method or its execution is off. This webinar will walk you through the most effective troubleshooting techniques chromatographers use to isolate problems in their systems, discuss proven injection and loading techniques and other best practices for optimizing chromatographic results, and for your longer-term success, also address important method development factors geared to extending the life of your column. - Recognizing problem indicators
- Troubleshooting HPLC methods and procedures
- Pump head problems and associated symptoms
- Detector and flow cell issues and corrective actions
- Sample injection problems
- Loading conditions to improve peak shape
- Sample injection techniques to help prevent blockages
- Tuning your methods to extend column life
Speaker: Todd Anderson, Global Chromatography Specialist, Teledyne ISCO
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Six new ideas for the successful purification of natural products, including Chinese Traditional Medicines
September 2023 If you use chromatography in the purification of natural products, including Chinese Traditional Medicines, you will want to watch this highly informative webinar. It’s practical information you can use immediately to work faster and get more accurate, consistent results.
During this you will learn: - What you need to do before processing your biomass.
- Wide-Polarity Range chromatography: Run columns and recover all the bioactive compounds.
- Extraction to purification: How to choose a column and elution solvents based on solvents used for extraction.
- How to use Assay Directed Fractionation with the Focus Gradient Generator to streamline your workflow for both western and eastern Traditional Medicines.
- Beyond silica: Alternative media useful for purifying natural products.
- Beyond flash chromatography: How Supercritical Fluid Chromatography can be used for natural products.
Speaker: Jack Silver, Teledyne ISCO Applications Chemist
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Introduction to Flash Chromatography: Top 5 Essentials to Maximize Success in Flash
July 2023 Flash chromatography has been around for decades and plays a critical role in pharmaceutical R&D labs for the purification of small molecules, peptides, and oligonucleotides because it is fast, easy to use and versatile. Flash is still a primary purification practice that continues to evolve and is as significant today as when it was introduced. New predictive method development tools are an important advance in improving flash chromatography by increasing resolution, decreasing solvents used, and increasing the number of successful purifications. During this you will learn: - Essentials for understanding how flash chromatography works and to enable you to successfully use it on regular basis.
- How flash chromatography has evolved to increase throughput and time-savings for scientists.
- Why you should switch to using an automated solution for flash chromatography if you are still using glass columns for your daily purifications.
- What new tools and features are available to expedite method development for flash chromatography, including examples of the advantage of using PeakTrak’s Focused Gradient Generator.
Speaker: Dylan Cavey, Teledyne ISCO Applications Chemist
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Preparative Supercritical Fluid Chromatography: Features and Benefits for the Modern Lab
June 2023 In this webinar, David Leach of
Reach Separations discusses the similarities and differences between HPLC and SFC processes and instruments and the benefits of switching to SFC. He describes different SFC workflows and specific hardware and software features to consider in a Prep SFC system and how Reach uses Teledyne ISCO’s ACCQPrep SFC. He also offers specifics on Reach’s work in HPLC and SFC for chiral and achiral purification and what positions Reach and its employees as experts in these techniques.
Leach has 15 years of experience in preparative purification and manages the flow of projects through the laboratories, designating scientists and equipment. He is responsible for investigating improvements to working practices through procedures or equipment investment.
Speaker: David Leach, Principal Separations Scientist, Reach Separations
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Scale up with RediSep: App Examples of scale up performance on 7.0 kg and 8.6 kg
June 2023 RediSep large columns will help you when tou need hundreds of grams, or even kilograms, of sample. This 45-minute webinar plus interactive Q&A, hosted by Teledyne ISCO application chemist and chromatography expert Jack Silver, will teach you how to develop methods for large columns, scale-up from smaller purifications, estimate how much can be loaded on a column, load samples, and how to use RediSep columns on any system, not just those from Teledyne ISCO.
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Do you want to take your Prep HPLC and SFC purifications to the next level?
April 2023 We want to help you get the most out of your Preparative HPLC and SFC system. During this 45-minute webinar plus interactive Q&A, Teledyne ISCO’s Global Chromatography Specialist, Todd Anderson, will discuss how to get the most out of your Preparative HPLC and SFC instrument, regardless of manufacturer. We discuss many topics that challenge chromatographers — everything from columns and mobile phase conditions to detection options, sample makeup and even safety considerations.
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Flash chromatography method development without TLC plates?! Is it possible?
March 2023 The answer may surprise you... The Flash Focus Gradient Generator delivers an optimized preparative method using the results of a scouting run quickly and more efficiently than TLC. The Flash Focus Gradient Generator is now a new feature in the CombiFlash® NextGen system that makes the calculations fast and easy. View the webinar to learn the advantages this new technique has over TLC method development.
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When Silica doesn't cut it for Flash Chromatography... what can I do?
February 2023 A majority of flash purifications are performed on bare silica with simple solvent systems. But when your first choice for a separation doesn’t work, what are your options for achieving a successful purification?
During this 45-minute webinar, we cover several alternative stationary phases to silica (C18, C18Aq, Amine, C8, Alumina) that are available for flash purifications. The webinar also covered application examples of their utility as well as different tools on the CombiFlash® NextGen chromatography units that enable the use of different solvent systems.
You’ll learn what range of stationary phases are available and how to choose appropriate stationary and mobile phases for tough separations. You’ll also learn how to use tools on the CombiFlash NextGen system that help expand the number of available solvents for flash separations and how to protect your investment with proper care of alternative stationary phase columns that are reusable, saving your lab money in the long run.
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How Can Stacked Injections Improve Throughput?
November 2022
Stacked injections can increase the amount of sample purified in a day. This is done by using the time needed for a peak to elute after injection to inject more sample, so that there are a series of injections going down the column.
Stacked injections are most useful when a compound needs to be resolved from a closely eluting impurity, whose presence requires a low column-loading to reduce overlapping peaks. Although this situation commonly occurs with chiral compounds, achiral purification such as peptides, natural products, and organic synthesis also benefit from stacked injections. PeakTrak software and the ACCQPrep SFC allow you to create stacked injections very easily!
During this 45-minute webinar, followed by an interactive Q&A session, you will learn:
- Why do stacked injections?
- What samples are amenable to stacked injections?
- Preparing your sample for stacked injections with your CombiFlash® NextGen flash chromatography system
- Setting up stacked injections on your ACCQPrep SFC system
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Chiral SFC Method Development on Polysaccharide-Based Chiral Stationary Phases- CTI
September 2022 Method development for chiral separations can be daunting for new and even experienced users. This 45-minute webinar plus Q&A session will cover the initial development and further optimization of chiral SFC methods using polysaccharide-based chiral stationary phases from Daicel.
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Using 7.0 / 9.0 kg RediSep® Gold Columns with the CombiFlash Torrent® for large-scale separations
October 2022 As newly created compounds find additional value during R&D, larger-scale synthesis and purifications are needed to meet increased demand. What began on the mg to g scale is now needed on the multi-gram to kg scale. during this 45-minute presentation, we will discuss how the Torrent and large RediSep prepacked columns are available to simplify scale-up to meet growing needs while increasing throughput and efficiency.
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Basics of Chromatography
July 2022: From this 45-minute presentation you will learn information that is applicable to preparative chromatography, analytical chromatography and all SFC (supercritical fluid chromatography), HPLC (high-performance liquid chromatography) or flash chromatography systems, no matter the manufacturer.
We’ll discuss common mistakes even seasoned chemists make when running TLC (thin layer chromatography) plates and offer solutions that will have you running TLC and other chromatography systems with greater confidence, speed and accuracy.
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Getting Started with your ACCQPrep HPLC System
June 2022 From this 45-minute presentation we will help you get up to speed faster and with confidence with your ACCQPrep HPLC System. If you aren’t used to preparative high-performance liquid chromatography systems, these useful tips and tactics will help you obtain successful, accurate, repeatable results.
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Getting Started with your CombiFlash System
May 2022
From this 45-minute presentation we will help you get up to speed faster and with confidence with your CombiFlash System. Users of other products will also learn useful tips and tactics for successful, accurate, repeatable results.
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Chromatography and Natural Products Purification
April 2022
From this 45-minute presentation plus interactive Q&A session, attendees will gain greater insight into the critical factors impacting the purification of natural products and how to gain accurate, repeatable results in a green, cost-efficient process.
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Large-scale Flash Separations: Scalability, Sample Loading and Method Development
March 2022
From this 45-minute presentation plus interactive Q&A session, attendees will gain greater insight into the critical factors impacting large-scale flash purifications and how to significantly reduce costs as scale increases.
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New Techniques for Prep SFC Method Development
February 2022 From this 45-minute presentation plus interactive Q&A session, attendees will be able to significantly reduce method development time for preparative SFC applications, while also saving solvent and other resources. Learn also how to access Teledyne ISCO’s chromatography support team at any time for assistance with Prep SFC instrument operation and methods.
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Preparative Supercritical Fluid Chromatography Made Easy
February 2022
From this 45-minute presentation plus interactive Q&A session, attendees will become fully oriented to the capabilities and key advantages of the ACCQPrep SFC system and its intended applications. Also learn how to access Teledyne ISCO’s chromatography support team at any time for assistance with Prep SFC instrument operation and methods.
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Introduction to Preparative Supercritical Fluid Chromatography
January 2022
From this 45-minute presentation plus interactive Q&A session, attendees will have a better understanding of the basics of Prep SFC and how and when to implement it for critical separations. Also learn how to access Teledyne ISCO’s chromatography support team at any time for assistance with Prep SFC instrument operation and methods.
Watch the Webinar |
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HPLC Troubleshooting and Maintenance
December 2021
From this 45-minute presentation plus interactive Q&A session, attendees will learn to spot common problems earlier and become faster and more confident in troubleshooting them. Labs will save time, money, and trouble by avoiding issues that cause columns to fail prematurely. Also learn how to access Teledyne ISCO chromatography specialists at any time to assist with your prep HPLC applications.
Speaker: Todd Anderson, Global Chromatography Specialist, Teledyne ISCO
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Purification and Isolation of Cannabinoids Other than CBD from Hemp
September 2021 From this 45-minute presentation plus interactive Q&A session, attendees will understand the most important issues impacting reverse-phase flash method development and how to resolve them quickly and effectively using the latest tools and best practices.
Speaker: Bryan Corey, Director of Science, Lab Society
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Reverse Phase Flash Method Development Made Easy
July 2021 From this 45-minute presentation plus interactive Q&A session, attendees will understand the most important issues impacting reverse-phase flash method development and how to resolve them quickly and effectively using the latest tools and best practices.
Speaker: Jack Silver, Applications Chemist
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HPLC Troubleshooting and Maintenance Techniques
June 2021
From this 45-minute presentation plus interactive Q&A session, attendees will learn to spot common problems earlier and become faster and more confident in troubleshooting them. Labs will save time, money, and trouble by avoiding issues that cause columns to fail prematurely. Also learn how to access Teledyne ISCO chromatography specialists at any time to assist with your prep HPLC applications.
Speaker: Todd Anderson, Global Chromatography Specialist, Teledyne ISCO
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Prep HPLC Simplified
May 2021 From this 45-minute presentation plus interactive Q&A session, attendees will learn to quickly identify the most important factors in prep HPLC method development and use them to more rapidly isolate target compounds with higher purity and greater yields.
Speaker: Todd Anderson, Global Chromatography Specialist, Teledyne ISCO
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Easy Prep Gradients from Analytical Runs
April 2021
From this 45-minute presentation plus interactive Q&A session, attendees will learn a variety of proven techniques for developing preparative chromatography methods, and best practices for focus gradient methods to purify target compounds quickly and more efficiently.
Speaker: Jack Silver, Application Chemist
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Discovery and Optimization of New Anti-migraine Peptides
April 2021 From this 45-minute presentation plus interactive Q&A session, attendees will gain stronger insight into the role of small protein molecules, how to better purify them with reverse phase flash chromatography methods, and how to quickly solve the most common method development problems.
Speaker(s): Dr. Christopher R. Coxon, Institute of Chemical Sciences, School of Engineering and Physical Sciences, Heriot-Watt University, Edinburgh, UK; Josh Lovell, Applications Specialist at Teledyne ISCO
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Large-Scale Flash Separations: Sample Loading, Method Development and Scalability Issues
March 2021 From this 45-minute presentation plus interactive Q&A session, attendees will gain greater insight into the critical factors impacting large-scale flash purifications and how to significantly reduce costs as scale increases
Speaker: Josh Lovell, Applications Specialist, Teledyne ISCO
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Flash Method Development from TLC Plates
February 2021
In this beginner-to-intermediate-level webinar, Jack Silver, Applications Chemist, describes proven procedures for using TLC results to develop flash chromatography methods.
Speaker: Jack Silver, Application Specialist
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Improving Prep HPLC
January 2021 In this webinar, Josh Lovell, Application Specialist, outlines the most important factors to consider in developing preparative LC methods.
Speaker: Josh Lovell, Application Specialist
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Introduction into Flash ChromatographyDecember 2020: In this webinar, Teledyne ISCO Application Chemist Josh Lovell gives us insight into flash chromatography, the similarities and differences of flash and HPLC and the benefits of automated flash chromatography. Speaker: Josh Lovell, Application Specialist
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Basics of Preparative ChromatographyNovember 2020: In our Chromatography webinar "Basics of Prep HPLC," we will discuss what Liquid Chromatography and HPLC are, the differences between analytical and prep, and the basic method development of isocratic vs. gradient. Other important topics to be covered include: effects of buffering and buffer choice for purification; injection solvent effects and solvent choices; solvent grade for prep and solvent selection; column care; and common troubleshooting techniques. Speaker: Jack Silver, Application Specialist
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Sample Loading Techniques for Flash and Prep HPLCOctober 2020: The choice of liquid vs. solid sample loading can have a significant impact on flash and prep chromatography results. Understanding the differences in peak resolution and separation speed is critical to knowing when to apply each. In this webinar Josh Lovell, Chromatography Applications Specialist, discusses the key factors to consider as illustrated by example chromatograms. Speaker: Josh Lovell, Application Specialist
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Managing Detection Parameters for Improved Peak DetectionSeptember 2020: In this intermediate-to-advanced webinar Jack Silver discusses how and when to use All-Wavelength Collection, baseline correction, and ESLD temperature settings to solve difficult applications. Speaker: Jack Silver, Application Specialist
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Prep HPLC and Column Choice OverloadJuly 2020: In this novice-to-intermediate-level webinar, Todd Anderson, Global Chromatography Specialist, explains his proven, easy-to-use process for column selection developed during 20 years of working with Prep HPLC applications in laboratories around the world.
Speaker: Todd Anderson, Global Chromatography Specialist
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Why TFA Alone Is Not Enough for Preparative LC
June 2020: In this intermediate-level webinar Teledyne ISCO Applications Chemist Jack Silver explains the impact of triflouroacetic acid on preparative LC separations and why it is not an optimum solution in some applications. Alternative methods for controlling pH to improve separations are discussed.
Speaker: Jack Silver, Application Specialist
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Peptides in a FlashJune 2020: In this intermediate-to-advanced webinar Jack Silver shares a wealth of practical insights developed over 30 years of experience with peptide purification applications in a broad range of time-and-cost-sensitive environments.
Speaker: Jack Silver, Application Specialist
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Improving Prep HPLCMay 2020: In this webinar, designed for all Chromatography scientists, Teledyne ISCO Application Chemist, Josh Lovell gives us insight into Prep HPLC, the options available to you, some challenges you could face, and troubleshooting techniques to overcome those challenges.
Speaker: Josh Lovell, Application Specialist
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Chromatography Troubleshooting
May 2020: In this webinar, designed for all Chromatography scientists, Jack Silver, Teledyne ISCO Application Chemist, focuses on basic troubleshooting methods that are applicable to all Prep Chromatography systems.
Speaker: Jack Silver, Application Specialist
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Green Solutions for Flash and Prep Chromatography
April 2020: Teledyne ISCO Application Chemist, Josh Lovell explains that the right system, with green choices during method development, save solvent and allow you to focus on other matters in the laboratory. Speaker: Josh Lovell, Application Specialist
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Peptide Purification Overview
March 2020: Application Chemist at Teledyne ISCO, Jack Silver shows us how we can save time and money and get a high-quality purification.
Speaker: Jack Silver, Application Specialist
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Mass Directed PurificationFebruary 2020: In this webinar, Global Chromatography Specialist at Teledyne ISCO, Todd Anderson shows us how a mass directed auto purification system works and how the range of detection methods work together to ensure you don’t ‘come up empty’ on your purification.
Speaker: Todd Anderson, Global Chromatography Specialist
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Improving Prep HPLC
January 2020: In this webinar, Teledyne ISCO Application Chemist, Josh Lovell gives us insight into Prep HPLC, the options available to you, some challenges you could face, and troubleshooting techniques to overcome those challenges.
Speaker: Josh Lovell, Application Chemist
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Introduction to Flash Chromatography
December 2019: In this webinar, Teledyne ISCO Application Chemist Josh Lovell gives us insight into flash chromatography, the similarities and differences of flash and HPLC and the benefits of automated flash chromatography.
Speaker: Josh Lovell, Application Chemist
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Sample Loading and Preparation Techniques
November 2019: Global Chromatography Specialist with Teledyne ISCO, Todd Anderson explains how with the right knowledge and equipment you can optimize efficiency, meet your purity needs and minimize sample loss and down time - saving both time and resources. Speaker: Todd Anderson, Global Chromatography Specialist
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Focus Gradient Generator
September 2019: In this webinar Jack Silver, Application Chemist at Teledyne ISCO will explain how gradient calculations can save time, trouble and solvents, all while improving your chromatography, but there are limitations to the gradient calculation methods. Jack Silver will also introduce us to a new gradient calculation method and explain how to apply it to your projects. Speaker: Jack Silver, Application Chemist
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Peptide Techniques for Prep HPLC
August 2019: Purifying your own peptides can save you time and money. Vendors can send unrefined and costly peptides in a matter of days, but 99% pure peptides can take a month or longer to be delivered. Using Teledyne ISCO's Flash Chromatography you can cut costs from your budget and cut weeks from achieving your objective. Speaker: Jack Silver, Application Chemist
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Green Solvent Solutions for Flash Chromatography
June 2019: In this webinar, we will discuss "Green" chemistry in the context of developing methods including importance of stationary phase choice, alternate green solvent choices, and getting things right the first time. Finally, we will show examples of some of the system features on the NextGen and how you can use them to minimize environmental impact while maximizing purification efficiency. Speaker: Joshua Lovell, Application Chemist
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Automated Gradient Optimization
May 2019: We will be discussing how chemists use scouting gradients to evaluate reactions and compound purity. These scouting gradients can be used to determine an efficient focus gradient for the desired compound. Topics covered will include techniques to create purification methods from focus gradients, and we will describe a new method allowing calculation of a focus gradient from a scouting run. Speaker: Jack Silver, Application Chemist
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ACCQPrep HP150 IntroductionApril 2019: We introduce our newest product: the ACCQPrep HP150! This latest innovation in Preparative HPLC systems does not follow the traditional path of an analytical system, but rather is intuitive and eliminates the unnecessary and complex integration parameters found in many of the HPLC systems in today's market. Plus, the ACCQPrep HP150 can be customized to meet your high performance purification needs.
By the end of this webinar, you will learn:
- What the ACCQPrep HP150 is and how it was designed with the ease of a Flash system in mind.
- How our automation options can be used to increase unattended purification throughput.
- What the variable options are for sample loading which were created to meet your sample volume needs.
- How the Focus Gradient Generator can generate a focused gradient to purify your target compound.
Speaker: Ronald Lewis, Product Line Manager - Chromatography Watch the Webinar |
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What to Do When Things Go Wrong in Prep ChromatographyMarch 2019: In our chromatography webinar, we will focus basic troubleshooting methods that are applicable to all Prep Chromatography systems. We will also take a look at retention time variations and how to handle them. Plus, we will take a look at other possible errors, such as poor peak shape, split peaks, and ghost peaks.
Speaker: Jack Silver, Application Chemist
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Tips and Techniques to Optimize ELSD and MS Based Purification
February 2019: In our Chromatography webinar, we will discuss the benefits of ELSD as an alternative detection method to UV. We will also provide example of several applications and classes of compounds where ELSD may be useful, and how to choose and change different ELSD experimental parameters. Plus we will explain the advantages of our integrated ELSD options for the ACCQPrep and NextGen Chromatography lines.
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Download Slides
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Introduction to Flash Chromatography
January 2019: This webinar focuses on a comparison of HPLC and Flash Chromatography. We will look at both similarities and differences of these two processes, cover how to transition from TLC or open column chromatography to flash, and the associated advantages of flash. Lab managers and bench chemists involved in organic synthesis, where milligrams to grams of material need purified, or who are new to or not currently using flash chromatography will benefit from information covered in this webinar.
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Basics of Prep HPLC
December 2018: In our Chromatography webinar “Basics of Prep HPLC," we will discuss what Liquid Chromatography and HPLC are, the differences between analytical & prep, and the basic method development of isocratic vs. gradient.
Other important topics to be covered include: effects of buffering and buffer choice for purification; injection solvent effects and solvent choices; solvent grade for prep and solvent selection; column care; and common trouble shooting techniques.
Watch the Webinar |
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Green Solvent Solutions for Flash ChromatographyNovember 2018: The NextGen series of systems are designed with "Green" chromatography in mind. Its methods offer faster separations with improved gradient profiles resulting in less solvent used. These savings decrease our environmental footprint while maximizing lab efficiency in both time and cost savings. New Features on the NextGen including baseline correction allows for greener, safer solvents not typically used in chromatography.
In this webinar, we will discuss green chemistry in the context of developing methods including importance of stationary phase choice, alternate green solvent choices, and getting things right the first time. Finally, we will show examples of some of the system features on the NextGen and how you can use them to minimize environmental impact while maximizing purification efficiency.
Watch the Webinar |
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Purification Considerations for PeptidesOctober 2018: In this webinar, we will also explore the advantages of mass-directed peptide purification and using flash chromatography in the pre-purification of peptides.
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Natural Product PurificationAugust 2018: Topics covered include: what to do before purification, “Wide-Polarity Range” chromatography, column screening using “Wide-Polarity Range” chromatography for method development, and specialty chromatography media for natural products including Sephadex, ion exchange, and polyamide resin. Although the topics discussed use automated chromatography systems, the techniques can be adapted to hand-packed columns. You will come away from this webinar with a better understanding of natural product purifications and how Teledyne ISCO can meet these needs.
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