Need a faster way to purify compounds
University of Liverpool, UK
The purification of organic compounds is a challenging and time-consuming process. More specifically, purification of UV inactive
compound is challenging through traditional chromatography techniques; different functional groups are active in other TLC stains
and may not give proper colour when your molecule is complex. I was working with peptides which are UV-inactive and challenging
to identify them on TLC and takes a lot of time to purify. Some of the peptides are not soluble in organic solvents and to purify such
compounds; we need C18 reversed-phase silica gel, purification is very slow and sometimes takes days. Waiting a long time to get
my compound purified was not feasible.
Robust and reliable solution for automated flash column chromatography
University of Bath, UK
We are an organic synthesis group working in the area of photoredox catalysis for amine synthesis. We needed a robust and reliable
solution for automated flash column chromatography, with an option for ELS detection to separate non-UV-active contaminants (i.e.,
tetrabutylammonium residues) from our products during reversed-phase chromatography.
High demand for flash systems resulting in delays
Sheffield Hallam University, UK
There was often a high demand for the flash systems that we have which would result in queues and delays. We wanted to purchase
a new flash system to alleviate this strain with the system being in budget, user friendly and cost effective, whilst delivering high levels
Increased need for high performance purification for impurity isolation
Global Pharmaceutical Company
Our department lacked capability and experience in Preparative chromatography. As the complexity and speed of our small molecule portfolio was evolving the demand for high performance chromatographic purification for impurity isolation increased.
Need a fast and consistent method for purification of a large range of organic mixtures
University of Edinburgh, UK
Our research is focused on the development and understanding of catalytic methods. During reaction optimization and determining
substrate scope and limitations we spend lots of time and energy purifying material, in the past we would do this by manual column
chromatography. We required a fast and consistent method for the purification of a large range of organic mixtures.
Replace old outdated systems
Bachem needed multiple prep HPLC systems to replace our old outdated systems. Our requirements included:
- Small footprint
- Integrated loading pump
- Capable of loading large volumes
- Mid-run auto-zero
- Fexible flow rate
- Easy to use
- Multiple wavelength
- Comparable or superior separation to our old systems and other systems on the market
Time-consuming and tedious purification step
Heriot-Watt University, UK
Our lab is interested in developing new chemical tools to understand, and ultimately treat, neurological and neurodegenerative diseases.
Our favourite way to do this is by designing and synthesising peptides that mimic parts of a protein or an endogenous peptide hormone.
We also typically add other non-native chemical modifications to these peptides e.g. fluorinated groups to help with our studies. In a
typical project, we will make hundreds of small peptides and sometimes we will need relatively large quantities (>100s of milligrams). However, the purification step can be a really time-consuming and tedious process.
Expand capabilities and make use of reverse phase conditions
Manchester Institute Drug Discovery Unit, UK
We had been using the CombiFlash® Rf and Rf+ units for several years using normal phase cartridges. We wanted to expand our
capabilities to be able to make use of reverse phase conditions on a scale larger than our existing preparative HPLC system but
did not really know where to start.
Need for minor training on instrument upkeep and maintenance
University of Greenwich, UK
We had only minor issues over the time such as “how to properly clean the ELSD detector” or “do we need to change the seals now?”
Simple and easy-to-use system capable of a variety of physical parameters
Durham University, UK
As an academic group working in synthetic methodology and chemical biology we need to purify compounds with a great variety
of physical parameters. Moreover the through put of people through the lab is large so any system has to be robust, simple to learn
and use and easy to maintain.
Perform routine purification operations quickly and effectively
University of Sunderland, UK
Our organic and medicinal chemistry research team at the University of Sunderland is a reasonably small team of researchers interested in natural product synthesis their biological applications, and drug discovery and design. While we have a number of post-graduate researchers working full-time in our purpose build synthetic organic chemistry laboratory, it was essential for academic staff with high teaching workloads, to be able to perform routine purification operations quickly and effectively.