​Teledyne ISCO Chromatography Webinars

Next Webinar

What to Do When Things Go Wrong in Prep Chromatography

Live Webinar | Tuesday, March 19, 2019 | 10:00 AM CDT
Speaker: Jack Silver, Application Chemist

In our chromatography webinar, we will focus basic troubleshooting methods that are applicable to all Prep Chromatography systems. We will also take a look at retention time variations and how to handle them. Plus, we will take a look at other possible errors, such as poor peak shape, split peaks, and ghost peaks.

​Previous Webinars

Utilizing ELSD for the Purification of Weak UV Absorbing Compounds

 In our Chromatography webinar, we will discuss the benefits of ELSD as an alternative detection method to UV. We will also provide example of several applications and classes of compounds where ELSD may be useful, and how to choose and change different ELSD experimental parameters. Plus we will explain the advantages of our integrated ELSD options for the ACCQPrep and NextGen Chromatography lines.

Download the presentation slides here.

Introduction to Flash Chromatography

This webinar focuses on a comparison of HPLC and Flash Chromatography. We will look at both similarities and differences of these two processes, cover how to transition from TLC or open column chromatography to flash, and the associated advantages of flash. Lab managers and bench chemists involved in organic synthesis, where milligrams to grams of material need purified, or who are new to or not currently using flash chromatography will benefit from information covered in this webinar.

Basics of Prep HPLC

In our Chromatography webinar “Basics of Prep HPLC," we will discuss what Liquid Chromatography and HPLC are, the differences between analytical & prep, and the basic method development of isocratic vs. gradient.

Other important topics to be covered include: effects of buffering and buffer choice for purification; injection solvent effects and solvent choices; solvent grade for prep and solvent selection; column care; and common trouble shooting techniques.

Green Solvent Solutions for Flash Chromatography 

The NextGen series of systems are designed with "Green" chromatography in mind. Its methods offer faster separations with improved gradient profiles resulting in less solvent used. These savings decrease our environmental footprint while maximizing lab efficiency in both time and cost savings. New Features on the NextGen including baseline correction allows for greener, safer solvents not typically used in chromatography.

In this webinar, we will discuss green chemistry in the context of developing methods including importance of stationary phase choice, alternate green solvent choices, and getting things right the first time. Finally, we will show examples of some of the system features on the NextGen and how you can use them to minimize environmental impact while maximizing purification efficiency.

Purification Considerations for Peptides

In this webinar, we will also explore the advantages of mass-directed peptide purification and using flash chromatography in the pre-purification of peptides.

Natural Product Purification

Topics covered include: what to do before purification, “Wide-Polarity Range” chromatography, column screening using “Wide-Polarity Range” chromatography for method development, and specialty chromatography media for natural products including Sephadex, ion exchange, and polyamide resin. Although the topics discussed use automated chromatography systems, the techniques can be adapted to hand-packed columns. You will come away from this webinar with a better understanding of natural product purifications and how Teledyne ISCO can meet these needs.

Introduction to Flash Chromatography

This webinar focuses on a comparison of HPLC and Flash Chromatography. We will look at both similarities and differences of these two processes, cover how to transition from TLC or open column chromatography to flash, and the associated advantages of flash. Lab managers and bench chemists involved in organic synthesis, where milligrams to grams of material need purified, or who are new to or not currently using flash chromatography will benefit from information covered in this webinar.

Improving Prep HPLC

In this webinar, we will present previous guidelines regarding sample loading based on loop size and what our actual experiments showed in order to maximize sample loading volume without sample loss to waste.  Also, we'll describe other tips to maximize injection efficiency and sample recovery. Finally, we will discuss how secondary methods of detection may be advantageous to various applications and things to consider when implementing. 

The Benefits of a Combined Flash/Prep System

This presentation discusses using a Flash system for routine purifications with the added convenience of having preparative HPLC capabilities. At times there are difficult separations where Flash simply does not have the necessary resolution. Other times compounds need purities in excess of 95% for library cataloging purposes.

​EZ Prep Introduction Webinar

Ron Lewis presents a webinar introducing the CombiFlash EZ Prep to Teledyne ISCO's Chromatography product line.

​Save Time and Money with EZ Prep Automation

Ron Lewis presents a webinar introducing the Automation Modules and how they can benefit customers that need to automate some of the mundane tasks involved with Flash and Prep chromatography.

​The Use of Alternative Media in Flash Chromatography

Jack Silver presents a webinar on the use of alternative media in flash chromatography. To purify compounds, most flash chromatography relies on silica gel and high performance C18 columns. In unique circumstances, these types of columns do not adequately purify compounds and may require alternative column chemistries. This webinar discusses alternative chemistries and the required method developments.