CombiFlash NextGen 300+

CombiFlash​® NextGen 300+

Fully Featured and Ready To Go

The CombiFlash NextGen 300+ features all available performance and safety options. Flow rate range is from 1 to 300 mL/min at operating pressures up to 300 psi (20 bar). The system is fitted with a solid load injection valve that can be bypassed if you decide to perform a liquid injection. RFID technology comes standard and automatically reads the size and type of RediSep or RediSep Gold® column being used for the purification. Based on this information, a default gradient and flow rate are programmed in​to the system. Simply insert the column into the stand, press Play and the run starts. This same RFID technology automatically reads the rack type being used ensuring there is never a missed or overfilled tube. Active solvent and waste level sensing, along with column air purge, is standard is standard on the NextGen 300+.



When you purchase instruments from Teledyne ISCO, you not only receive innovative, high quality products, you also receive excellent customer service and product support. Your initial purchase is only the beginning of Teledyne ISCO's ongoing commitment to their customers.


CombiFlash NextGen Quick Start GuideQuick Start Guide
CombiFlash NextGen Site PreparationTechnical Note
CombiFlash NextGen User ManualUser Manual
Purification of Delicate Compounds with RediSep Rf Gold Diol and Cyano ColumnsApp Note
Time-On-Target Easy Method Development for Reverse Phase Preparative ChromatographyPoster
CombiFlash Pump Seal Replacement InstructionsInstruction Sheet
CombiFlash NextGen 300+ DatasheetDatasheet
CombiFlash NextGen Safety SheetInstruction Sheet
CombiFlash NextGen300 Prolonged ShutdownTechnical Note
Instructions for Large Column StandInstruction Sheet
Optimized Peptide PurificationBrochure
CombiFlash System Verification InstructionsInstruction Sheet
Purification of Peptide-Peptoid Hybrids App NoteApp Note
CombiFlash NextGen BrochureBrochure
Manual vs Automated Flash Chromatography, Part 2App Note
Manual vs Automated Flash Chromatography, Part 1App Note
Flash Chromatography Method Development - in a Flash!Poster


​CombiFlash NextGen 300+ Specifications

​Flow Rate Range
​1 - 300 mL/min
​Pressure Limit
​300 psi (20 Bar)
​Column Sizes
​4 - 750 g
Sample Size ​10 mg - 30 g
​User Interface

​12 in touchscreen (30.5 cm)

15 in touchscreen (38.1 cm)

​Dimensions (H x W x D) 26 x 14.1 x 17 in (66 x 36 x 43 cm)​

​For a full list of product specifications, see the datasheet.




  • Straightforward
    • Complete control on one screen
  • Compact
    •  Designed to take up​ minimal lab space
  • Flexible
    •  Configure to meet your specific needs
  • Faster
    •  New default methods speed up the flow without sacrificing performance
  • Greener
    •  Conserve solvent with optimized gradients


Standard Features

  • Flow rates from 1 to 300 mL/min
  • Operating pressure up to 300 psi (20 Bar)
  • Choice of UV, UV-Vis ELSD, or MS options
  • Active solvent and waste level sensing
  • Air purge
  • TUV Certified​