ACCQPrep® HP150

High Pressure Preparative Liquid Chromatography System

The Teledyne ISCO ACCQPrep HP150 was developed and designed with the ease of a Flash system in mind. It does not follow the traditional path of an analytical system, but rather this system has been designed to be intuitive and easy-to-use.

The ACCQPrep HP150 eliminates the unnecessary and complex integration parameters found in many of the HPLC systems in today's market. Our PeakTrak® software, which currently drives our CombiFlash® product line, is the basis for controlling the ACCQPrep HP150. By using this software, the need for a standalone PC is eliminated. This results in a smaller, more compact unit with an integrated touch screen interface.

Enjoy the confidence associated with Teledyne ISCO's active solvent and waste level monitoring which reduces the risk of solvent spills. In addition, all sample and collection racks are RFID coded eliminating the potential of missed tubes.
An exciting feature of the ACCQPrep HP150 is the Focus Gradient Generator. When activated, the user is prompted to run a preset scouting gradient then they select the target peak for purification and the focused gradient is automatically programmed into the system.

The ACCQPrep HP150 is compatible with Teledyne ISCO Automation Modules and other detection systems for maximum efficiency.

 Overview


​Standard Features

  • ​Flow rates from 1 to 150 mL/min allow development of Prep methods directly on the system without the need for pump head changes
  • Operating pressure up to 6000 psi
  • Choice of UV or UV-Vis plus ELSD and MS options


 


Compatible with Automation Modules

  • Use the AutoInjector Module to make multiple purification runs on the​ same sample
  • Use the AutoSampler Module if you have several different samples to purify which may require multiple injections and different chromatographic conditions
  • Use the Column Selector Module to automatically switch to the next column required for your purification

Compatible with PurIon Mass Spectrometer

  • Add mass directed fractionation to your purification
  • Use with Teledyne ISCO's PeakTrak software for single point control of all mass spectrometer parameters
  • Choose from three models to meet the needs of your application

 Specifications

​​​​​ACCQ​Prep HP 150

​Flow Rate Range ​1 - 150 mL/min
​Pressure Limit ​up to 6000 psi (400 bar) Lower pressure limits user defined
​UV and UV-Vis Detection ​​Variable UV 200 - 400 nm standard, Variable UV-Vis 200 - 800 nm option
​ELSD Detection ​Integrated Option that can be combined with either UV or UV-Vis
​Number of Solvents ​​Binary gradient. 2 solvent std.  2x3 solvents optional
​Gradient Formation
​High Pressure
​Programmable gradients ​Linear, step, isocratic
​Gradient Linearity ​+/- 1%
​Sample Introduction ​Variable volume liquid load (5mL loop standard)
​User Interface ​15 in touchscreen
​Fraction Collector ​Internal
​Dimensions​ ​27.5 x 14.0 x 20.0 in (69.9 x 35.6 x 50.8 cm) (H x W x D)
84.5 pounds (38.2 kg)
​Dimensions AS 2x2 Autosampler Module ​​18.9 x 14.0 x 19.3 in (48.0 x 35.6 x 49.0 cm)​ (H x W x D)
31.1 lbs (14.1 kg)
​Dimensions AS 4x2 Autosampler Module ​27.5 x 27.5 x 22 in (69.9 x 58.4 x 55.9 cm)​ (H x W x D)
31.1 lbs (14.1 kg)

For a full list of product specifications, see the datasheet.

Datasheet

 Videos

 
 


 Documents

  
  
Chimeric Diol Column Behavior using Aqueous Solvents App NoteApp Note
Effects of Reverse Phase Chain Length on Peptide PurificationApp Note
Purification of a Peptide ACE Inhibitor App NoteApp Note
ACCQPrep HP150 BrochureBrochure
ACCQPrep HP150 DatasheetDatasheet
ACCQPrep Column Selector Instruction SheetInstruction Sheet
ACCQPrep Injection Valve Rotor ReplacementInstruction Sheet
ACCQPrep Preventative Maintenance Instruction SheetInstruction Sheet
ACCQPrep Verification Instruction SheetInstruction Sheet
ELSD Air Pump Instruction SheetInstruction Sheet
Installing the ACCQPrep AI 5 AutoInjector Instruction SheetInstruction Sheet
A Method to Save Time and Solvents Generating Purification Methods from Scouting GradientsPoster
Fall ACS 2017 Flow Chemistry PosterPoster
Fall ACS 2017 Pore Size PosterPoster
On-Column Dilution A Technique to Load Samples Dissolved in DMSOPoster
Time-On-Target Easy Method Development for Reverse Phase Preparative ChromatographyPoster
ACCQPrep HP150 Site PrepTechnical Note
ACCQPrep Minimum Injection VolumeTechnical Note
ACCQPrep Sample Loop Maximum Injection VolumeTechnical Note
Choosing the Right Purification SystemTechnical Note
Chromatography Equipment Installation and Operation ChecklistTechnical Note
Exporting and Quantitating Chromatography Data from the ACCQPrep HP125Technical Note
Guide to Different Loading Methods for the ACCQPrepTechnical Note
Maximizing Sample Recovery on the ACCQPrepTechnical Note
Normal Phase Purifications with the ACCQPrepTechnical Note
Scouting Pause I Why Use A Scouting PauseTechnical Note
Scouting Pause II Determination of Maximum Sample LoadTechnical Note
ACCQPrep HP125 HP150 User ManualUser Manual

 Support

When you purchase instruments from Teledyne ISCO, you not only receive innovative, high quality products, you also receive excellent customer service and product support. Your initial purchase is only the beginning of Teledyne ISCO's ongoing commitment to their customers.

​ACCQPrep HP150

Preparative Liquid Chromatography

The Teledyne ISCO ACCQPrep HP150 was developed and designed with the ease of a Flash system in mind. It does not follow the traditional path of an analytical system, but rather this system has been designed to be intuitive and easy-to-use.

The ACCQPrep HP150 eliminates unnecessary and complex integration parameters found in many of the HPPLC systems in today’s market. Our PeakTrak® software, which currently drives our CombiFlash® product line, is the basis for controlling the ACCQPrep HP150. By using this software, the need for a standalone PC is eliminated. This results in a smaller, more compact unit with an integrated touch screen interface.

Enjoy the confidence associated with Teledyne ISCO’s active solvent and waste level monitoring, which reduces the risk of solvent spills. In addition, all sample and collection racks are RFID coded eliminating the potential of missed tubes.

The ACCQPrep HP150 uses two dual piston reciprocating pumps with high pressure mixing to minimize gradient delay volumes. With high pressure gradient formation and a unique pump design which operates across the full range of flow rates from analytical (1 mL/min) to 50 mm and larger preparative columns (150 mL/min), the need to switch out pump heads is eliminated. This allows the use of an analytical column for simplified scouting and method development directly on the system

  • Straightforward
    • Complete control of the system on one screen
  • Compact
    • Designed to take up minimal lab space
  • Flexible
    • Add on Teledyne ISCO Automation Modules and other sample injection and detection options for maximum efficiency

 

Standard Features:

  • Flow rates from 1 to 150 ml/min allow development of Prep methods directly on the system
  • Operating pressure up to 6,000 psi
  • Choice of UV or UV-Vis plus ELSD and MS options
  • Focused Gradient Generator