Teledyne ISCO Universal Test Mix (phenacetin and N-benzylbenzamide) was run (image below).
Image above: Universal
Test Mix runs with TLC plates from different vendors.
The plates were run in 50:50 hexanes/ethyl acetate v/v. Not only are the predicted column volumes different, but the relative spot movements are different. The variation in relative retention affects loading estimates.
Teledyne ISCO Universal Test Mix was run on TLC plates from different manufacturers. The variation between different TLC plates is even more pronounced with this mixture.
Teledyne ISCO TLC plates provide accurate retention and loading data for method development and are easier to use.