Mass-directed Purification of Steroids with APCI and CombiFlash PurIon App NoteApp Note
PurIon BrochureBrochure
CombiFlash RfPlus PurIon DatasheetDatasheet
PurIon Installation ProcedureInstruction Sheet
CombiFlash RfPlus PurIon System Verification Technical NoteLegacy Document
Networking Guidelines For Combiflash Products Technical NoteTechnical Note
PurIon Flash Mass Installation Requirements Technical NoteTechnical Note
CombiFlash User Manual (Excluding Torrent)User Manual


When you purchase instruments from Teledyne ISCO, you not only receive innovative, high quality products, you also receive excellent customer service and product support. Your initial purchase is only the beginning of Teledyne ISCO's ongoing commitment to their customers.


​CombiFlash® Rf+ PurIon

Flow Rate Range​1 - 200 mL/min
​Pressure Limit
200 psi (13.8 bar)
​UV and UV- Vis Detection
​Variable UV 200 - 400 nm standard. Variable UV-Vis 200 - 800 nm option
​ELSD Detection​Option that can be combined with either UV or UV-Vis
​Mass Spectrometry Detection​50 – 1200 Dalton, 1 Dalton Resolution 

Electrospray Ionization (ESI) or optional Atmospheric Pressure Chemical Ionization (APCI)

Positive or Negative Ionization Mode

​SIM Sensitivity​10 pg Reserpine, 100:1 S/N (RMS) with SIM of m/z
​Number of SolventsBinary gradient with any two of four solvents; A third solvent can be used to add modifier at a fixed percentage.
​Programmable Gradients​Linear, step, isocratic
​Sample Injection​Direct
​User Interface​10.4" touchscreen
​Fraction Collector​Internal
​Dimensions (H x W x D)
​CombiFlash® Rf - 24 x 14 x 17 in (60 x 36 x 43 cm);
Mass spectrometer - 26 x 11 x 22 in (66 x 28 x 56 cm);
Roughing pump - 10 x 9 x 18 in​ (26 x 23 x 46 cm)​

​For a full list of product specifications, see the datasheet.



​​CombiFlash® Rf+ PurIon

Verify before you purify

The CombiFlash Rf+ PurIon takes the guesswork out of your purification routine with mass directed purification. Identify your peak of interest at the time of elution with the advantage of targeted fraction collection. Simply instruct the Flash system to collect only those peaks matching the mass of your target compound, eliminating the need for further analysis and saving time in post-purification processing. 

As with all other CombiFlash models, Teledyne ISCO's intuitive PeakTrak® software lets you access all the commands you need from a single touchscreen. The method development screen facilitates confirmation of the detectable ions of your product, and helps you identify and set appropriate detection parameters prior to the purification process.

Standard Features

  • Real-time method editing and control 
  • Touch-and-drag gradients 
  • Real-time and post-run MS and UV spectral display 
  • Flow rates to 200 mL/min at pressures up to 200 psi (13.8 bar) 
  • Compatible with column sizes from 4 to 330 grams 
  • Mass range from 50 to 1200 Dalton with 1 Dalton resolution 
  • Use with your choice of ELSD, UV, or UV- Vis detectors​