Chromatography

​Teledyne ISCO Chromatography Webinars

Next Webinar

Green Solvent Solutions for Flash Chromatography

Live Webinar | Thursday, November 15, 2018 | 10:00 AM CDT
Speaker: Joshua Lovell, Application Chemist

 

The NextGen series of systems are designed with "Green" chromatography in mind. Its methods offer faster separations with improved gradient profiles resulting in less solvent used. These savings decrease our environmental footprint while maximizing lab efficiency in both time and cost savings. New Features on the NextGen including baseline correction allows for greener, safer solvents not typically used in chromatography.

In this webinar, we will discuss green chemistry in the context of developing methods including importance of stationary phase choice, alternate green solvent choices, and getting things right the first time. Finally, we will show examples of some of the system features on the NextGen and how you can use them to minimize environmental impact while maximizing purification efficiency.

​Upcoming Webinars

Basics of Prep HPLC

Live Webinar | Thursday, December 6, 2018 | 10:00 AM CDT
Speaker: Jack Silver, Application Chemist

 

In our Chromatography webinar “Basics of Prep HPLC," we will discuss what Liquid Chromatography and HPLC are, the differences between analytical & prep, and the basic method development of isocratic vs. gradient.

Other important topics to be covered include: effects of buffering and buffer choice for purification; injection solvent effects and solvent choices; solvent grade for prep and solvent selection; column care; and common trouble shooting techniques.

​Previous Webinars

Purification Considerations for Peptides

In this webinar, we will also explore the advantages of mass-directed peptide purification and using flash chromatography in the pre-purification of peptides.

Natural Product Purification

Topics covered include: what to do before purification, “Wide-Polarity Range” chromatography, column screening using “Wide-Polarity Range” chromatography for method development, and specialty chromatography media for natural products including Sephadex, ion exchange, and polyamide resin. Although the topics discussed use automated chromatography systems, the techniques can be adapted to hand-packed columns. You will come away from this webinar with a better understanding of natural product purifications and how Teledyne ISCO can meet these needs.

Introduction to Flash Chromatography

This webinar focuses on a comparison of HPLC and Flash Chromatography. We will look at both similarities and differences of these two processes, cover how to transition from TLC or open column chromatography to flash, and the associated advantages of flash. Lab managers and bench chemists involved in organic synthesis, where milligrams to grams of material need purified, or who are new to or not currently using flash chromatography will benefit from information covered in this webinar.

Improving Prep HPLC

In this webinar, we will present previous guidelines regarding sample loading based on loop size and what our actual experiments showed in order to maximize sample loading volume without sample loss to waste.  Also, we'll describe other tips to maximize injection efficiency and sample recovery. Finally, we will discuss how secondary methods of detection may be advantageous to various applications and things to consider when implementing. 

Streamline Peptide Synthesis and Purification

The increased use of peptides as active site ingredients and Active Pharmaceutical Ingredients (APIs) necessitates improved purification techniques. This webinar will address how to handle impurities in synthesized peptides which often result from contaminants in the protected amino acid reagents and incomplete reactions as the peptide chain is lengthened.

The Benefits of a Combined Flash/Prep System

This presentation discusses using a Flash system for routine purifications with the added convenience of having preparative HPLC capabilities. At times there are difficult separations where Flash simply does not have the necessary resolution. Other times compounds need purities in excess of 95% for library cataloging purposes.

EZ Prep Introduction Webinar

Ron Lewis presents a webinar introducing the CombiFlash EZ Prep to Teledyne ISCO's Chromatography product line.

Save Time and Money with EZ Prep Automation

Ron Lewis presents a webinar introducing the Automation Modules and how they can benefit customers that need to automate some of the mundane tasks involved with Flash and Prep chromatography.

The Use of Alternative Media in Flash Chromatography

Jack Silver presents a webinar on the use of alternative media in flash chromatography. To purify compounds, most flash chromatography relies on silica gel and high performance C18 columns. In unique circumstances, these types of columns do not adequately purify compounds and may require alternative column chemistries. This webinar discusses alternative chemistries and the required method developments.

Introducing the CombiFlash Rf+ PurIon

Ron L. Lewis presents a webinar on Teledyne Isco's CombiFlash Rf+ PurIon Flash Chromatography and Mass Spectrometry system. This presentation outlines the benefits of mass-directed compound purification with the CombiFlash Rf+ PurIon.

Sample Loading Techniques in Flash Chromatography

Ron L. Lewis presents a webinar on solid versus liquid sample loading. Ron provides details on selecting the best loading technique for your sample and identifies potential pitfalls with each technique. This presentation is a practical session with examples of what works and what does not.

Greener Flash Chromatography Using C18 Columns

Ron L. Lewis presents the benefits of using C18 column media with reversed phase flash chromatography. Benefits include column reuse, higher loading, less solvent usage, and a smaller carbon footprint.